TY - JOUR AU - Zobi, Fabio AU - Obst, Martin PY - 2017/02/22 Y2 - 2024/03/29 TI - Two- and Three-dimensional Mid-Infrared Chemical Imaging JF - CHIMIA JA - Chimia VL - 71 IS - 1-2 SE - Scientific Articles DO - 10.2533/chimia.2017.32 UR - https://www.chimia.ch/chimia/article/view/2017_032 SP - 32 AB - This article describes the state-of-the-art in 2D and 3D tomographic mid-IR imaging and its current and potential applications in the imaging of material and biological systems with a focus on cells and tissues. 2D FTIR microscopy is first presented in terms of the basic experimental configurations of the technique, optics, data and image acquisition. This first section provides a basis for the ensuing discussion on 3D FTIR spectro-microtomography. In the second part, examples of 2D and 3D chemical imaging are given with a focus on the use of molecular chemical probes and probe-free applications, respectively. ER -