@article{Steffen_Börner_Freisinger_Sigel_2019, title={Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET}, volume={73}, url={https://www.chimia.ch/chimia/article/view/2019_257}, DOI={10.2533/chimia.2019.257}, abstractNote={ Exploring the spatiotemporal dynamics of biomolecules on a single-molecule level requires innovative ways to make them spectroscopically visible. Fluorescence resonance energy transfer (FRET) uses a pair of organic dyes as reporters to measure distances along a predefined biomolecular reaction coordinate. For this nanoscopic ruler to work, the fluorescent labels need to be coupled onto the molecule of interest in a bioorthogonal and site-selective manner. Tagging large non-coding RNAs with single-nucleotide precision is an open challenge. Here we summarize current strategies in labeling riboswitches and ribozymes for fluorescence spectroscopy and FRET in particular. A special focus lies on our recently developed, DNA-guided approach that inserts two fluorophores through a stepwise process of templated functionality transfer and click chemistry. }, number={4}, journal={CHIMIA}, author={Steffen, Fabio D. and Börner, Richard and Freisinger, Eva and Sigel, Roland K. O.}, year={2019}, month={Apr.}, pages={257} }