Continuous Micro-Production Using Enzymatic Reaction and Online Monitoring

Authors

  • Regula Moser Ecole d’ingénieurs et d’architectes de Fribourg Boulevard de Pérolles 80;
  • Thierry Chappuis Ecole d’ingénieurs et d’architectes de Fribourg Boulevard de Pérolles 80
  • Ennio Vanoli Ecole d’ingénieurs et d’architectes de Fribourg Boulevard de Pérolles 80
  • Simon Crelier Ecole d’ingénieurs et d’architectes de Fribourg Boulevard de Pérolles 80
  • Olivier Naef Ecole d'ingénieurs et d'architectes de Fribourg Boulevard de Pérolles 80 CP 32 CH-1705 Fribourg;, Email: olivier.naef@hefr.ch

DOI:

https://doi.org/10.2533/chimia.2010.799

Keywords:

Chymotrypsin, Enzyme immobilization, Enzymatic reaction, Micro-production, Micro-reactor

Abstract

A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit® C, controlled-pore glass (CPG), and Sepharose.
CHIMIA Vol. 64 No. 11 (2010): Swiss Biotech

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Published

2010-11-26

Issue

Vol. 64 No. 11 (2010): Swiss Biotech

Section

Scientific Articles

License

Copyright (c) 2010 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
R. Moser, T. Chappuis, E. Vanoli, S. Crelier, O. Naef, Chimia 2010, 64, 799, DOI: 10.2533/chimia.2010.799.