Enantioselective Switch and Potential Applications in Biocatalysis

Authors

  • Lucia Robustini Dept. Chemistry, Biochemistry and Pharmaceutical Sciences. University of Bern, Freiestrasse 3, CH-3012 Bern
  • Francesca Paradisi Dept. Chemistry, Biochemistry and Pharmaceutical Sciences. University of Bern, Freiestrasse 3, CH-3012 Bern https://orcid.org/0000-0003-1704-0642

DOI:

https://doi.org/10.2533/chimia.2023.390

PMID:

38047778

Keywords:

Biocatalysis, Enantiopreference, Enzymes, Transaminase

Abstract

Enantioselectivity has always been a key feature of enzymatic synthesis. In some cases, when enzymes are not strictly enantioselective, by tuning the reaction conditions it is possible to induce an enantioselective switch. A transaminase from Halomonas elongata (ω-HeWT), while generally S-selective, could be shifted towards generating the R-enantiomer at higher concentrations of amino acceptor or ionic strength, for example. Other enzymes are reported to have a similar behavior, and here we discuss some of them and their potential applications.

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Published

2023-06-28

Issue

Section

Scientific Articles