Challenges in ESI-MS-based Untargeted Metabolomics

Authors

  • Elena Tobolkina School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland
  • Víctor González-Ruiz School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland; Swiss Centre for Applied Human Toxicology (SCAHT), Switzerland
  • Isabel Meister School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland
  • Miguel De Figueiredo School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland
  • Davy Guillarme School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland
  • Julien Boccard School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland; Swiss Centre for Applied Human Toxicology (SCAHT), Switzerland
  • Serge Rudaz School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland; Swiss Centre for Applied Human Toxicology (SCAHT), Switzerland

DOI:

https://doi.org/10.2533/chimia.2022.90

PMID:

38069754

Keywords:

Annotation, Capillary electrophoresis, Liquid chromatography, Mass spectrometry, Metabolomics, Supercritical fluid chromatography, Toxicological and doping analysis

Abstract

Untargeted metabolomics is now widely recognized as a useful tool for exploring metabolic changes taking place in biological systems under different conditions. In this article, we aim to provide a short overview of the liquid-phase separation methods hyphenated to MS to perform untargeted metabolomics of biological samples. Each approach is complemented by up-to-date literature to guide readers, as well as practical information for avoiding or fixing some of the most frequently encountered pitfalls. This article covers mainly data acquisition, but a short discussion is provided regarding signal processing and data treatment, as well as data analysis and its biological interpretation in the context of metabolomic studies.

CHIMIA Vol. 76 No. 1-2 (2022): Mass Spectrometry at Swiss Academic and Industrial Institutions

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Published

2022-02-23

Issue

Vol. 76 No. 1-2 (2022): Mass Spectrometry at Swiss Academic and Industrial Institutions

Section

Scientific Articles

License

Copyright (c) 2022 Serge Rudaz, Elena Tobolkina, Víctor González-Ruiz, Isabel Meister, Miguel De Figueiredo, Davy Guillarme, Julien Boccard

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

[1]
E. Tobolkina, González-Ruiz V., I. Meister, M. De Figueiredo, D. Guillarme, J. Boccard, S. Rudaz, Chimia 2022, 76, 90, DOI: 10.2533/chimia.2022.90.