Polyhistidine Affinity Chromatography: From the Idea to a Method of Broad Applicability and the Pitfalls in-between

Authors

  • Heinz Döbeli Lupsingerstrasse 22, CH-4417 Ziefen, Switzerland;, Email: heinz.doebeli@bman.ch

DOI:

https://doi.org/10.2533/chimia.2020.363

PMID:

32482212

Keywords:

Affinity tag removal, Histidine affinity tag, Nickel chelate, Ni-nta, Protein folding, Protein purification

Abstract

The present article recapitulates the development of the polyhistidine affinity tag purification principle. Emphasis is laid on events behind the scenes which were never published. The key concept of the method emerged in a team discussion and its further development was driven by the need of Roche in-house projects.
CHIMIA Vol. 74 No. 05(2020): Building Bridges between Biotechnology and Chemistry : In Memoriam Oreste Ghisalba

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Published

2020-05-27

Issue

Vol. 74 No. 5 (2020): Building Bridges between Biotechnology and Chemistry: In Memoriam Oreste Ghisalba

Section

Scientific Articles

License

Copyright (c) 2020 Heinz Döbeli

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
H. Döbeli, Chimia 2020, 74, 363, DOI: 10.2533/chimia.2020.363.