PeBiToSens™: A Platform for PBT Screening of Fragrance Ingredients Without Animal Testing

Authors

  • Heike Laue Fragrances S&T, Givaudan Schweiz AG, Kemptpark 50, CH-8310 Kemptthal, Switzerland;, Email: heike.laue@givaudan.com
  • Lu Hostettler Fragrances S&T, Givaudan Schweiz AG, Kemptpark 50, CH-8310 Kemptthal, Switzerland
  • Gordon Sanders Regulatory Affairs and Product Safety, Givaudan International SA, 5, Chemin de la Parfumerie, CH-1214 Vernier, Switzerland
  • Georg Kreutzer Regulatory Affairs and Product Safety, Givaudan International SA, 5, Chemin de la Parfumerie, CH-1214 Vernier, Switzerland
  • Andreas Natsch Fragrances S&T, Givaudan Schweiz AG, Kemptpark 50, CH-8310 Kemptthal, Switzerland;, Email: andreas.natsch@givaudan.com

DOI:

https://doi.org/10.2533/chimia.2020.168

PMID:

32197676

Keywords:

Acute aquatic toxicity, Bioaccumulation, Biodegradation, Ecotoxicity, In vitro

Abstract

The determination of persistence (P), bioaccumulation (B) and toxicity (T) plays a central role in the environmental assessment of chemicals. Persistence is typically evaluated via standard microbial biodegradation tests. Bioaccumulation refers to the accumulation of chemicals in organisms and is usually assessed in fish exposed to the test chemical. Toxicity is determined at three trophic levels, with fish toxicity as the highest trophic level assessed. Thus, animal tests are classically needed for both B and T assessment. In vitro systems based on fish liver cells or liver S9 fractions ('RT-S9 assay') have been recently adopted by OECD to measure the biotransformation rates for the chemicals for B assessment. Biotransformation drives clearance from the body and reduces bioaccumulation. For T assessment, an assay based on in vitro toxicity on fish gill cells has been established ('RTgill-W1 assay'). Here we summarize our findings indicating that these tests are highly predictive for fragrance ingredients, and show with two case studies of our latest new registered substances how we apply these tests in particular during development and also for chemical registration. This platform of tests (PeBiToSens™) could fully replace animal tests in ecotoxicological assessment and is key in the Givaudan Safe by Design™ approach to develop safer and environmentally compatible novel fragrance ingredients.

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Published

2020-03-25