Artificial Lysosomal Platform to Study Nanoparticle Long-term Stability

Authors

  • Ana Milosevic Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg; Swiss Federal Laboratories for Materials Science and Technology (Empa), Lerchenfeldstrasse 5, CH-9014 St. Gallen
  • Joël Bourquin Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg
  • David Burnand Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg; Federal Department of Economic Affairs, Education, and Research, EAER, Laboratory of ‘Proteins & Metabolites’, Agroscope, Schwarzenburgstr. 161, CH-3003 Bern
  • Philipp Lemal Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg
  • Federica Crippa Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg
  • Christophe A. Monnier Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg; Waite Research Lab, Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, USA
  • Laura Rodriguez-Lorenzo Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg; INL – International Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga 4715-330 Braga, Portugal
  • Alke Petri-Fink Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg; Department of Chemistry, University of Fribourg, Chemin du Musee 9, CH-1700 Fribourg
  • Barbara Rothen-Rutishauser Adolphe Merkle Institute, Faculty of Science and Medicine, University of Fribourg, Chemin des Verdiers 4, CH-1700 Fribourg

DOI:

https://doi.org/10.2533/chimia.2019.55

PMID:

30813999

Keywords:

Artificial lysosomal fluids, Cell uptake, Engineered nanoparticles, Lysosomes, Stability

Abstract

Nanoparticles (NPs) possess unique properties useful for designing specific functionalities for biomedical applications. A prerequisite of a safe-by-design and effective use in any biomedical application is to study NP–cell interactions to gain a better understanding of cellular consequences upon exposure. Cellular uptake of NPs results mainly in the localization of NPs in the complex environment of lysosomes, a compartment which can be mimicked by artificial lysosomal fluid. In this work we showed the applicability of lysosomal fluid as a platform for a fast assessment of gold, iron oxide and silica NP stability over 24 h in a relevant biological fluid, by using multiple analytical methods.

CHIMIA Vol. 73 No. 01-02(2019): NCCR Bio-Inspired Materials

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Published

2019-02-27

Issue

Vol. 73 No. 1-2 (2019): NCCR Bio-Inspired Materials

Section

Scientific Articles

License

Copyright (c) 2019 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
A. Milosevic, J. Bourquin, D. Burnand, P. Lemal, F. Crippa, C. A. Monnier, L. Rodriguez-Lorenzo, A. Petri-Fink, B. Rothen-Rutishauser, Chimia 2019, 73, 55, DOI: 10.2533/chimia.2019.55.