Structure and Sedimentation Kinetics of Dense Suspensions of Fibroblast Cells

Abstract

We investigate the structure and the dynamics of dense suspensions of NIH 3T3 fibroblast cells. Using two-photon microscopy we obtain three dimensional (3D) images from which the size and the packing structure of the dense cell suspensions can be extracted. In addition, we analyse the global time-dependent behaviour of the suspensions by time-lapse measurements of cell sedimentation. Since cell adhesion is a non-equilibrium living process the interplay can be influenced by cell viability interfering with cell–cell interactions.