Platforms for Bioorthogonal Oligonucleotide-templated Reactions: Translating Concepts into Devices

Authors

  • Suraj Pavagada Department of Bioengineering Imperial College London London SW7 2AZ, UK
  • Sylvain Ladame Department of Bioengineering Imperial College London London SW7 2AZ, UK. sladame@imperial.ac.uk

DOI:

https://doi.org/10.2533/chimia.2018.809

Keywords:

Oligonucleotide-templated reaction, Sensing, Hydrogel, Diagnostic, Paper-microfluidic

Abstract

The exponential improvements made in DNA sequencing technologies, together with the rapidly declining associated costs, has increasingly led to the expansion of the field of personalised genomic medicine. Changes in the sequence or copy number of specific deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules represent key signatures for the diagnosis, prognosis, classification and monitoring of a broad range of pathologies, most notably cancer. Technologies that can detect these changes require analytical tools that can detect DNA or RNA with high sensitivity and high specificity. Sensing based on bioorthogonal oligonucleotide-templated reactions (OTRs) has been recognised as an elegant strategy that satisfies these criteria and was successfully used for the quantitative detection of nucleic acids both in vitro and in vivo. Herein, we will focus on recent efforts to implement bioorthogonal OTRs into clinically useful biosensors using probes immobilised on or embedded in customised materials and platforms.

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Published

2018-11-30

How to Cite

[1]
S. Pavagada, S. Ladame, Chimia 2018, 72, 809, DOI: 10.2533/chimia.2018.809.

Issue

Section

Scientific Articles