Automated Microscopy and High Content Screens (Phenotypic Screens) in Academia Labs

Authors

  • Dimitri Moreau ACCESS-Geneva, NCCR Chemical-Biology, Department of Biochemistry, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland. Dimitri.Moreau@unige.ch
  • Jean Gruenberg ACCESS-Geneva, NCCR Chemical-Biology, Department of Biochemistry, University of Geneva, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland. jean.gruenberg@unige.ch

DOI:

https://doi.org/10.2533/chimia.2016.878

Keywords:

High-content screening, Image-based screening

Abstract

Imaged-based screening has been developing extremely quickly in the past 10 years. Academic institutes quickly realized that the discovery capacity of this technology was huge, allowing the automatic detection and quantification of complex cell phenotypes. Associated with chemical or genetic perturbations, high content screening is the method of choice for a deep system biology analysis. The evolution of high-content screening is mainly due to the recent progress in the development of fast and high quality automated imagers and of a plethora of new very bright fluorescent markers, so that almost any cellular element can be seen and imaged. In this paper we review and summarize the major steps in the development of an image-based screening project.

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Published

2016-12-21

How to Cite

[1]
D. Moreau, J. Gruenberg, Chimia 2016, 70, 878, DOI: 10.2533/chimia.2016.878.