Potassium Sensitive Optical Nanosensors Containing Voltage Sensitive Dyes

Authors

  • Xiaojiang Xie Department of Inorganic and Analytical Chemistry, University of Geneva Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland
  • Agustín Gutiérrez Department of Inorganic and Analytical Chemistry, University of Geneva Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland
  • Valentin Trofimov Department of Biochemistry, University of Geneva Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland
  • Istvan Szilagyi Department of Inorganic and Analytical Chemistry, University of Geneva Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland
  • Thierry Soldati Department of Biochemistry, University of Geneva Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland
  • Eric Bakker Department of Inorganic and Analytical Chemistry, University of Geneva Quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland. Eric.Bakker@unige.ch

DOI:

https://doi.org/10.2533/chimia.2015.196

Keywords:

Nanosphere, Optical sensor, Potential, Valinomycin, Voltage sensitive dye

Abstract

Ionophore-based ion-selective optical nanosensors have been explored for a number of years. Voltage sensitive dyes (VSDs) have been introduced into this type of sensors only very recently, forming a new class of analytical tools. Here, K+-sensitive nanospheres incorporating a lipophilic VSD were successfully fabricated and characterized. The nanosensors were readily delivered into the social amoeba Dictyostelium discoideum in a non-invasive manner, forming a promising new platform for intracellular ion quantification and imaging.

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Published

2015-04-29