Comparison of UHPLC-ESI-MS and Hadamard Transform Atmospheric Pressure Ion Mobility-ESI-MS for Rapid Profiling of Isomeric Flavonoids

Michael Groessl; Antonio Azzollini; Philippe J. Eugster; Benoit Plet; Jean-Luc Wolfender; Richard Knochenmuss

doi:10.2533/chimia.2014.135

Comparison of UHPLC-ESI-MS and Hadamard Transform Atmospheric Pressure Ion Mobility-ESI-MS for Rapid Profiling of Isomeric Flavonoids

Authors

Michael Groessl Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland
Antonio Azzollini School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
Philippe J. Eugster School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
Benoit Plet Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland
Jean-Luc Wolfender School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
Richard Knochenmuss Tofwerk AG, Uttigenstrasse 22, CH-3600 Thun, Switzerland

DOI:

https://doi.org/10.2533/chimia.2014.135

Keywords:

Esi-tof-ms, Flavonoids, Glycosides, Ion mobility, Uhplc

Abstract

Hadamard transform atmospheric pressure ion mobility-MS and rapid UHPLC-MS methods were investigated for analysis of closely related isomeric flavonoids and their glycosides using a test set of seven standards. On a time scale of a few minutes, the flavonoid aglycones were all separated by ion mobility, but not by UHPLC. The glycosides were better resolved by IMS but not completely separated by both methods. The results suggest that IMS provides sufficient resolution for separation of isomeric polyphenols such as flavonoids in high-throughput metabolomics studies.