Kinetic Subpopulations Detected by Single-molecule Spectroscopy: Fundamental Property of Functional Nucleic Acids or Experimental Artefact?
DOI:
https://doi.org/10.2533/chimia.2013.240Keywords:
Heterogeneity, Group ii introns, Memory effect, Smfret, SplicingAbstract
Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1* interaction, two RNA sequences that play an important role in group II intron mediated self-cleavage. Further examples of dynamic heterogeneity in functional nucleic acids are provided and the possible origins of this phenomenon are discussed.Downloads
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2013-04-24
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Scientific Articles
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Copyright (c) 2013 Swiss Chemical Society
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How to Cite
[1]
S. L. B. König, D. Kowerko, R. K. O. Sigel, Chimia 2013, 67, 240, DOI: 10.2533/chimia.2013.240.