Construction of a Peptide Microarray for Auto-anti- body Detection

FH - HES

Authors

  • Vincent Cosandey HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
  • Fabien Debrot Institute of Chemistry, University of Applied Sciences Western Switzerland, Freiburg, Perolles 80, CH-1705 Freiburg, Switzerland
  • Jérémy Kaeser HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
  • Roger Marti Institute of Chemistry, University of Applied Sciences Western Switzerland, Freiburg, Perolles 80, CH-1705 Freiburg, Switzerland
  • Philippe Passeraub Institute of Sciences and Industrial Technologies, University of Applied Sciences Western Switzerland, Geneva, 4 rue de la Prairie, CH-1202 Geneva, Switzerland
  • Jannick Pétremand HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
  • Denis Prim HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland
  • Marc E. Pfeifer HES-SO Valais, Institute of Life Technologies, Route du Rawyl 47, CH-1950 Sion 2, Switzerland. marc.pfeifer@hevs.ch

DOI:

https://doi.org/10.2533/chimia.2012.803

Keywords:

Bard1, Click chemistry, Microarray, Peptide antigens, Tumor auto-antibodies

Abstract

Peptide and protein microarrays provide a multiplex approach to identification and quantification of protein–protein interactions (PPI), useful to study for instance antigen–antibody properties. Multivariate serology assays detecting multiple tumor auto-antibodies (TAA) is an emerging class of blood tests for cancer detection. Here we describe the efficient coupling of peptide baits derived from the BRCA1-associated RING domain protein 1 (BARD1) to a solid surface and detection of a commercially available anti-BARD1 antibody with this newly designed peptide microarray. Analytical sensitivity and specificity were shown to be comparable to a microtiter plate based enzyme-linked immunosorbent assay (ELISA).
CHIMIA Vol. 66 No. 10 (2012): NMR Spectroscopy in Switzerland

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Published

2012-10-31

Issue

Vol. 66 No. 10 (2012): NMR Spectroscopy in Switzerland

Section

Columns, Conference Reports

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License

Copyright (c) 2012 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
V. Cosandey, F. Debrot, J. Kaeser, R. Marti, P. Passeraub, J. Pétremand, D. Prim, M. E. Pfeifer, Chimia 2012, 66, 803, DOI: 10.2533/chimia.2012.803.