Visualizing Biochemical Activities in Living Cells through Chemistry


  • Luc Reymond
  • Rudolf Griss
  • Alberto Schena
  • Birgit Mollwitz
  • Karolina Bojkowska
  • Anastasiya Masharina
  • Matthias A. Brun
  • Damien Maurel
  • Keitaro Umezawa
  • Gražvydas Lukinavičius
  • Kai Johnsson Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland



Cell biology, Imaging, Protein chemistry, Snap-tag, Synthetic probes


The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over the last years, our laboratory has developed different labeling approaches for monitoring protein activity and for localizing synthetic probes inside living cells. In this article, we review two of these labeling approaches, the SNAP-tag and CLIP-tag technologies, and their use for studying cellular processes.




How to Cite

L. Reymond, R. Griss, A. Schena, B. Mollwitz, K. Bojkowska, A. Masharina, M. A. Brun, D. Maurel, K. Umezawa, G. Lukinavičius, K. Johnsson, Chimia 2011, 65, 868, DOI: 10.2533/chimia.2011.868.



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