Screening of Large Molecule Diversities by Phage Display

Authors

  • Inmaculada Rentero
  • Christian Heinis Ecole Polytechnique Fédérale de Lausanne, EPFL Institute of Chemical Sciences and Engineering, CH-1015 Lausanne, Switzerland. christian.heinis@epfl.ch

DOI:

https://doi.org/10.2533/chimia.2011.843

Keywords:

Antibody, Combinatorial library, Cyclic peptide, Directed evolution, Peptide, Phage display, Protein scaffold

Abstract

Molecules with tailored binding specificities are needed for many purposes such as the development of therapeutics, the detection and purification of biomolecules or in chemical biology for the study and manipulation of biological systems. With phage display technology, polypeptides with binding affinities to targets of interest can be isolated from billions of polypeptide variants with a modest amount of effort, time and cost. The technology was initially used for the generation and screening of peptide and antibody libraries and was later applied to many different protein scaffolds. More recently, chemically and structurally diverse molecule libraries were generated by chemically modifying phage-displayed polypeptides. In this article, the different classes of natural and non-natural structures that can be encoded and screened by phage display are reviewed with a special focus on bicyclic peptides that we routinely generate in our laboratory.

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Published

2011-11-23

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Scientific Articles