Searching for the Protein Targets of Bioactive Molecules

Authors

  • Christopher Chidley
  • Hirohito Haruki
  • Miriam Grønlund Pedersen
  • Cindy Fellay
  • Simone Moser
  • Kai Johnsson Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne

DOI:

https://doi.org/10.2533/chimia.2011.720

Keywords:

Sulfasalazine, Target deconvolution, Tetrahydrobiopterin, Yeast three-hybrid screening

Abstract

The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reveal physiologically relevant interactions. Here we review a recently introduced yeast-based approach for the identification of the binding partners of small molecules. We discuss the advantages and limitations of the approach using the clinically approved drug sulfasalazine as an example.

Downloads

Published

2011-09-30

Issue

Section

Scientific Articles