Searching for the Protein Targets of Bioactive Molecules

Authors

  • Christopher Chidley
  • Hirohito Haruki
  • Miriam Grønlund Pedersen
  • Cindy Fellay
  • Simone Moser
  • Kai Johnsson Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne

DOI:

https://doi.org/10.2533/chimia.2011.720

Keywords:

Sulfasalazine, Target deconvolution, Tetrahydrobiopterin, Yeast three-hybrid screening

Abstract

The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reveal physiologically relevant interactions. Here we review a recently introduced yeast-based approach for the identification of the binding partners of small molecules. We discuss the advantages and limitations of the approach using the clinically approved drug sulfasalazine as an example.

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Published

2011-09-30

How to Cite

[1]
C. Chidley, H. Haruki, M. G. Pedersen, C. Fellay, S. Moser, K. Johnsson, Chimia 2011, 65, 720, DOI: 10.2533/chimia.2011.720.

Issue

Section

Scientific Articles