High-Resolution and Tandem Mass Spectrometry – the Indispensable Tools of the XXI Century

Authors

  • Yury O. Tsybin Biomolecular Mass Spectrometry Laboratory, Ecole Polytechnique Fédérale de Lausanne, EPFL LSMB BCH 4307, CH-1015 Lausanne
  • Luca Fornelli
  • Anton N. Kozhinov
  • Aleksey Vorobyev
  • Sasa M. Miladinovic

DOI:

https://doi.org/10.2533/chimia.2011.641

Keywords:

Fourier transform ion cyclotron resonance, ft-icr, Liquid chromatography, Mass spectrometry, ms, Peptide, Petroleomics, Protein, Proteomics, Tandem mass spectrometry, ms/ms

Abstract

Mass spectrometry-based qualitative and quantitative (bio)molecular analysis is a corner stone in the state-of-the-art pipelines in systems biology and environmental sciences. High-resolution and efficient tandem mass spectrometry methods and techniques are the essential analytical capabilities for the in-depth analysis of extremely complex mixtures of (bio)molecules of a very broad dynamic range of concentrations. Here, we briefly review the advantages and limitations of the current mass spectrometry with a focus on resolution, or resolving power, and methods of (bio)molecular fragmentation in the gas phase. We conclude with an outlook that considers possible avenues for further mass spectrometry-based method and technique development, indispensable for advancing the challenging real-life mass spectrometry applications in the XXI century.

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Published

2011-09-30

Issue

Section

Scientific Articles

How to Cite

[1]
Y. O. Tsybin, L. Fornelli, A. N. Kozhinov, A. Vorobyev, S. M. Miladinovic, Chimia 2011, 65, 641, DOI: 10.2533/chimia.2011.641.