Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Authors

  • Jean-Luc Wolfender Phytochimie et Produits Naturels Bioactifs, Ecole de Pharmacie Genève-Lausanne, Section des Sciences Pharmaceutiques, Université de Genève Quai Ansermet 30, 1211 Genève 4;, Email: jean-luc.wolfender@unige.ch
  • Philippe J. Eugster Phytochimie et Produits Naturels Bioactifs, Ecole de Pharmacie Genève-Lausanne, Section des Sciences Pharmaceutiques, Université de Genève Quai Ansermet 30, 1211 Genève 4
  • Nadine Bohni Phytochimie et Produits Naturels Bioactifs, Ecole de Pharmacie Genève-Lausanne, Section des Sciences Pharmaceutiques, Université de Genève Quai Ansermet 30, 1211 Genève 4
  • Muriel Cuendet Phytochimie et Produits Naturels Bioactifs, Ecole de Pharmacie Genève-Lausanne, Section des Sciences Pharmaceutiques, Université de Genève Quai Ansermet 30, 1211 Genève 4

DOI:

https://doi.org/10.2533/chimia.2011.400

Keywords:

Biological activity, Metabolomics, Microflow nmr, Nutraceuticals, Uhplc-tof-ms

Abstract

Advances in analytical methods and bioassay development have helped to push forward the research in natural products. In plant extracts and nutraceuticals, bioactive compounds are part of a complex mixture. The development of high-resolution methods related to HPLC for both chemical and biological profiling has significantly increased the efficiency of classical bioactivity-guided fractionation procedures. Furthermore, the level of sensitivity obtained by these methods give the possibility to work with few micrograms of compound. This represents a key advantage for rapid localisation of the biological activity and subsequent identification of the compounds of interest. The same methods are also used to study the extracts from a metabolomic view point. The possibility to study them as a whole can highlight synergetic effects, which are likely to occur in plant extracts and nutraceuticals. In this paper, the main trends are summarised and the developments made in our laboratory on profiling crude extracts with UHPLC-TOF-MS, natural product identification at the microgram level using microflow NMR and integration of these methods with biological evaluation are highlighted.

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Published

2011-06-29