Single-Run Separation of Closely Related Cationic and Anionic Compounds by CE-ESI-MS: Application to the Simultaneous Analysis of Melamine and its Analogs in Milk

Authors

  • Isabelle Kohler School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Bd d'Yvoy 20, CH-1211, Geneva 4, Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4
  • Emmanuelle Cognard Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4, Food Authority Control of Geneva, Quai Ernest-Ansermet 22, CP 76, CH-1211 Geneva 4
  • Ivano Marchi Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4, Food Authority Control of Geneva, Quai Ernest-Ansermet 22, CP 76, CH-1211 Geneva 4
  • Didier Ortelli Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4, Food Authority Control of Geneva, Quai Ernest-Ansermet 22, CP 76, CH-1211 Geneva 4
  • Patrick Edder Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4, Food Authority Control of Geneva, Quai Ernest-Ansermet 22, CP 76, CH-1211 Geneva 4
  • Jean-Luc Veuthey School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Bd d'Yvoy 20, CH-1211, Geneva 4, Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4
  • Serge Rudaz School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Bd d'Yvoy 20, CH-1211, Geneva 4, Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4
  • Julie Schappler School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Bd d'Yvoy 20, CH-1211, Geneva 4, Swiss Centre for Applied Human Toxicology, University of Geneva, CMU, Rue Michel-Servet 1, CH-1211 Geneva 4;, Email: julie.schappler@unige.ch

DOI:

https://doi.org/10.2533/chimia.2011.389

Keywords:

Ce-ms, Cyanuric acid, Food analysis, Melamine, Single run

Abstract

In recent years, two adulteration incidents concerning the addition of melamine, a nitrogen-rich-industrial small polar compound, to pet food and infant formula products have occurred in China. These issues prompted laboratories to develop methods for the analysis of melamine and related compounds in a wide variety of food products and ingredients. In this context, a CE-ESI-MS method was developed to simultaneously analyze melamine and its related products (ammeline, ammelide and cyanuric acid) that possess close physico-chemical properties. This method allows the simultaneous analysis of both cations and anions in a single run, using CE to divide the run into two time segments in normal polarity mode. For this purpose, ESI polarity was switched once during the run, increasing sensitivity and data quality. The method was applied to spiked powdered milk and melamine-contaminated powdered milk, with two sample preparation procedures.
CHIMIA Vol. 65 No. 6 (2011): Nutrition Chemistry in Switzerland

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Published

2011-06-29

Issue

Vol. 65 No. 6 (2011): Nutrition Chemistry in Switzerland

Section

Scientific Articles

License

Copyright (c) 2011 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
I. Kohler, E. Cognard, I. Marchi, D. Ortelli, P. Edder, J.-L. Veuthey, S. Rudaz, J. Schappler, Chimia 2011, 65, 389, DOI: 10.2533/chimia.2011.389.