Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Authors

  • Sören Werner Zürcher Hochschule für Angewandte Wissenschaften Institut für Biotechnologie CH-8820 Wädenswil
  • David Hacker École Polytechnique Fédérale de Lausanne Faculty of Life Sciences, Laboratory of Cellular Biotechnology CH-1015 Lausanne; ExcellGene CH-1870 Monthey
  • Lucia Baldi École Polytechnique Fédérale de Lausanne Faculty of Life Sciences, Laboratory of Cellular Biotechnology CH-1015 Lausanne
  • Rolf Tanner ExcellGene CH-1870 Monthey
  • Markus Kühner Adolf Kühner AG CH-4127 Birsfelden
  • Gilles Broccard École Polytechnique Fédérale de Lausanne Faculty of Life Sciences, Laboratory of Cellular Biotechnology CH-1015 Lausanne
  • Cedric Bürkie ExcellGene CH-1870 Monthey
  • Stephanie Tissot École Polytechnique Fédérale de Lausanne Faculty of Life Sciences, Laboratory of Cellular Biotechnology CH-1015 Lausanne
  • Matthieu Stettler ExcellGene CH-1870 Monthey
  • Xiaowei Zhang École Polytechnique Fédérale de Lausanne Faculty of Life Sciences, Laboratory of Cellular Biotechnology CH-1015 Lausanne; Nanchang University Institute of Translational Medicine 330031, Nanchang, Jiangxi, PR China
  • Maria De Jesus ExcellGene CH-1870 Monthey
  • Dieter Eibl Zürcher Hochschule für Angewandte Wissenschaften Institut für Biotechnologie CH-8820 Wädenswil
  • Marcel Röll Sartorius Stedim Switzerland AG CH-8317 Tagelswangen
  • Christine Lettenbauer Sartorius Stedim Switzerland AG CH-8317 Tagelswangen
  • Regine Eibl Zürcher Hochschule für Angewandte Wissenschaften Institut für Biotechnologie CH-8820 Wädenswil
  • Florian M. Wurm École Polytechnique Fédérale de Lausanne Faculty of Life Sciences, Laboratory of Cellular Biotechnology CH-1015 Lausanne; ExcellGene CH-1870 Monthey;, Email: florian.wurm@epfl.ch

DOI:

https://doi.org/10.2533/chimia.2010.819

Keywords:

Disposable bioreactors, Engineering characteristics, High density cho cultures, Orbitally shaken, Wave-mixed

Abstract

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for the suspension cultures of cells, especially when combined with disposable materials. This article presents an overview of the engineering characteristics when these new principles are applied in bioreactors, and case studies covering scales of operation from milliliters to 1000 liters.
CHIMIA Vol. 64 No. 11 (2010): Swiss Biotech

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Published

2010-11-26

Issue

Vol. 64 No. 11 (2010): Swiss Biotech

Section

Scientific Articles

License

Copyright (c) 2010 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
S. Werner, D. Hacker, L. Baldi, R. Tanner, M. Kühner, G. Broccard, C. Bürkie, S. Tissot, M. Stettler, X. Zhang, M. De Jesus, D. Eibl, M. Röll, C. Lettenbauer, R. Eibl, F. M. Wurm, Chimia 2010, 64, 819, DOI: 10.2533/chimia.2010.819.