IMAPlate Based Miniature, High Sensitive, Rapid Screening Method for Detecting Bioengineered, Secreted Lipase Activities in Yeast Expression Systems
DOI:
https://doi.org/10.2533/chimia.2010.789Keywords:
Enzymatic assay, Imaplate, Miniaturized reactor, Protein expression, ScreenAbstract
A spectrophotometric assay based on a miniaturized 96-well plate device (IMAPlate) enables a rapid and simple screening of bioengineered recombinant lipases expressed and secreted by Saccharomyces cerevisiae. Starting from a colony, the test delivers a quantitative estimation of enzymatic activity titer in 24 h or less with manual high throughput performances.Downloads
Published
2010-11-26
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Scientific Articles
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Copyright (c) 2010 Swiss Chemical Society
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How to Cite
[1]
M. A. Sciotti, G. J. Chen, D. Gygax, Chimia 2010, 64, 789, DOI: 10.2533/chimia.2010.789.