Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium

Authors

  • Silvan Eichenberger
  • Laurent Bigler
  • Stefan Bienz

DOI:

https://doi.org/10.2533/chimia.2007.161

Keywords:

Liquid chromatography, Polyamine alkaloids, Spider venom, Structure elucidation, Tandem mass spectrometry

Abstract

The lyophilized venom of the spider Larinioides folium (Araneidae) was analyzed by high-performance liquid chromatography, coupled on-line to electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (HPLC-ESI-MS/MS), as well as UV-DAD analysis. In combination with amino acid analysis, high-resolution mass spectrometry and on-column H/D-exchange experiments, the structures of 41 new acyl-polyamines contained in the complex venom of the spider L. folium were elucidated. Data interpretation in detail and the efficiency of this combined set of analytical method is exemplified by the structural elucidation of one of the toxins, namely of LF503a.

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Published

2007-04-25