Structure Elucidation of Polyamine Toxins in the Venom of the Spider Larinioides folium
DOI:
https://doi.org/10.2533/chimia.2007.161Keywords:
Liquid chromatography, Polyamine alkaloids, Spider venom, Structure elucidation, Tandem mass spectrometryAbstract
The lyophilized venom of the spider Larinioides folium (Araneidae) was analyzed by high-performance liquid chromatography, coupled on-line to electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (HPLC-ESI-MS/MS), as well as UV-DAD analysis. In combination with amino acid analysis, high-resolution mass spectrometry and on-column H/D-exchange experiments, the structures of 41 new acyl-polyamines contained in the complex venom of the spider L. folium were elucidated. Data interpretation in detail and the efficiency of this combined set of analytical method is exemplified by the structural elucidation of one of the toxins, namely of LF503a.Downloads
Published
2007-04-25
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Scientific Articles
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Copyright (c) 2007 Swiss Chemical Society
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How to Cite
[1]
S. Eichenberger, L. Bigler, S. Bienz, Chimia 2007, 61, 161, DOI: 10.2533/chimia.2007.161.