Preparation of Modified RNA Sequences for Biological Research

Authors

  • Stefan Pitsch
  • Philipp Wenter
  • Alfred Stutz
  • Luc Reymond
  • Sébastien Porcher
  • Andreas Peer
  • Evelyne Muller
  • Muthulaniappan Meyyappan
  • Frédéric Meylan
  • Cyrille Denarie
  • Damian Ackermann
  • Xiaolin Wu

DOI:

https://doi.org/10.2533/000942905777675633

Keywords:

Ligation, Nucleic acids, Organic synthesis, Phosphoramidites, Rna

Abstract

Our group is working on the development of reliable access to biologically relevant, long, and modified RNA sequences. Here, we briefly present the chemical synthesis of such sequences with 2?-O-triisopropylsilyloxymethyl (= 2?-O-tom) protected ribonucleoside phosphoramidites, their template-directed enzymatic ligation, and some examples of modified nucleotides, designed to promote structural and biological studies.

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Published

2005-11-01

Issue

Vol. 59 No. 11 (2005): Modified Oligonucleotides

Section

Scientific Articles

License

Copyright (c) 2005 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
S. Pitsch, P. Wenter, A. Stutz, L. Reymond, S. Porcher, A. Peer, E. Muller, M. Meyyappan, F. Meylan, C. Denarie, D. Ackermann, X. Wu, Chimia 2005, 59, 808, DOI: 10.2533/000942905777675633.