Covalent Labeling of Fusion Proteins with Chemical Probes in Living Cells

Authors

  • Susanne Gendreizig
  • Antje Keppler
  • Alexandre Juillerat
  • Thomas Gronemeyer
  • Horst Pick
  • Horst Vogel
  • Kai Johnsson

DOI:

https://doi.org/10.2533/000942903777679433

Keywords:

O6-alkylguanine-dna alkyltransferase, Fluorescent probes, Protein labeling

Abstract

A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl group from O6-alkylguanine-DNA to one of its cysteine residues. Labeling is possible in bacterial as well as eukaryotic cells and is independent of the nature of the label, thereby opening up new ways to study proteins in vivo.

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Published

2003-04-01

Issue

Vol. 57 No. 4 (2003): Laureates: Awards and Honors (SCS)

Section

Scientific Articles

License

Copyright (c) 2003 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
S. Gendreizig, A. Keppler, A. Juillerat, T. Gronemeyer, H. Pick, H. Vogel, K. Johnsson, Chimia 2003, 57, 181, DOI: 10.2533/000942903777679433.