NMR Study of the p-DNA Duplex [(4→?2')-3'-Desoxyribopyranosyl-(ᵐ⁵CGDDTTᵐ⁵CG)₂] and Comparison with its p-RNA Analogue

Authors

  • Marc-Olivier Ebert Department of Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zürich
  • Damian Ackermann Department of Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zürich; Laboratoire de chimie des acides nucléiques, Ecole Polytechnique Fédérale Lausanne, EPFL, Bâtiment UNIL-BCH, CH-1015 Lausanne
  • Stefan Pitsch Department of Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zürich; Laboratoire de chimie des acides nucléiques, Ecole Polytechnique Fédérale Lausanne, EPFL, Bâtiment UNIL-BCH, CH-1015 Lausanne
  • Bernhard Jaun Department of Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, CH-8093 Zürich

DOI:

https://doi.org/10.2533/chimia.2001.852

Keywords:

p-DNA, Duplex formation, NMR solution structure, Pentapyranosyl nucleic acids, p-RNA

Abstract

The determination of the solution structure of small non-natural oligopeptides and oligonucleotides by NMR, which is one of the main research topics of our group, is illustrated on the example of an 8-mer p-DNA duplex. p-DNA, the 3'-desoxy analogue of p-RNA, forms an highly selective pairing system but its pairing strength is less than that of analogous p-RNA sequences. The NMR study reveals that the backbone of p-DNA corresponds more closely to the conformation predicted for pentapyranose nucleic acids by qualitative conformational analysis than p-RNA.

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Published

2001-10-31

Issue

Vol. 55 No. 10 (2001): The Department of Chemistry, ETH Zürich, in the New Hönggerberg Buildings

Section

Scientific Articles

License

Copyright (c) 2001 Swiss Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

[1]
M.-O. Ebert, D. Ackermann, S. Pitsch, B. Jaun, Chimia 2001, 55, 852, DOI: 10.2533/chimia.2001.852.