Development of Artificial Ribonucleases Using Macrocyclic Lanthanide Complexes

Abstract

The sequence-specific recognition and cleavage of ribonucleic acids (RNA) represents a fundamental challenge. Metal complexes are among the best synthetic catalysts for the cleavage of RNA. Covalent attachment of suitable macrocyclic lanthanide complexes to oligonucleotides allows the cleavage of complementary RNAs in a sequence-specific manner. The preparation, properties and use of such artificial ribonucleases are highlighted. In particular, the design and preparation of constructs cleaving RNA with multiple turn-over is described.