Enzymatic Synthesis of β-D-Glucuronides in an Enzyme Membrane Reactor

Abstract

The production of two O-glucuronides in an enzyme membrane reactor on a 100 to 200 mg scale was examined. The aglycons were conjugated with the co-substrate β-D-uridine diphosphoglucuronic acid (UDPGA) in the presence of a guinea-pig liver preparation. The continuous synthesis, which was run in an enzyme membrane reactor, was followed depending on the substrate up to 118 hours or 24 hours, respectively. The reaction was monitored by TLC or HPLC. The purification of the two glucuronides was carried out by ion-exchange chromatography and by reversed-phase HPLC.