Production with Bacterial and Mammalian Cells – Some Experiences

Abstract

Bridging the gap between academia and industry, between basic and applied research, was one of the targets of the 1^st Czech-Swiss Symposium on Advanced Biotechnology held in Prague, September 4–7, 1999. This presentation identifies some selected areas in industrial biotechnology, where we see potential for improvement. Our proposals are based on our experience with biotechnological processes, such as immobilised enzyme processes, whole cell fermentation processes, and mammalian cell culture, with products ranging from optically pure chemicals to monoclonal antibodies and proteins, and from our alliance with Genzyme Transgenics for the isolation and purification of selected proteins from transgenic animals. Since strain and process design for fermentation are key issues, and downstream processing (DSP) is dependent on steps upstream, mainly fermentation, we will focus our discussion on the fermentation step.