Preparation of Biotinylated and FITC-Labelled Phosphorylcholine Poly(acrylamide) Derivatives and Their Application for Protein Ligand-Binding Studies

Authors

  • Jiří Liberda
  • Tomáš Trnka
  • Jan Sejbal
  • Věra Jonáková
  • Marek Kraus
  • Marie Tichá

DOI:

https://doi.org/10.2533/chimia.1999.528

Abstract

Biotin- and FITC-labelled water-soluble poly(acrylamide) derivatives of phosphorylcholine were prepared by coupling either maleinylated (a) or periodate-oxidized (b) L-glyceryl phosphorylcholine to poly-(acrylamide-allylamine) copolymer. Biotinylated phosphorylcholine poly(acrylamide) derivatives of both types were tested with Limulus polyphemus C-reactive protein and were used for the study of the phosphorylcholine-binding properties of boar seminal plasma proteins. Binding sites for phosphorylcholine on the surface of bull sperms were visualized using a FITC-labelled derivative of the ligand.

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Published

1999-11-24

Issue

Section

Scientific Articles

How to Cite

[1]
J. Liberda, T. Trnka, J. Sejbal, V. Jonáková, M. Kraus, M. Tichá, Chimia 1999, 53, 528, DOI: 10.2533/chimia.1999.528.