Isolierung, HPLC-Trennung und Quantifizierung der Sesquiterpenfraktion von Petasites hybridus (L.) G. M. et SCH.

Abstract

A simple and easily reproducible HPLC procedure allows to separate total extracts of Petasites hybridus (L.) G. M. et SCH. and to detect numerous sesquiterpenes (in most cases sesquiterpene esters) of the general formula 1 (isopetasols), 2 (neopetasols), 3 (petasols), 4 (13-substituted isopetasols), and 5 (13-substituted neopetasols). Four so far unknown sesquiterpene esters 2f (neo-S-petasin), 2g ((E)-3-(methylthio)-acryloyl-neopetasol), 3g ((E)-3-(methylthio)-acryloyl-petasol), and 5b (3-desoxy-13-(angeloyloxy)-neopetasol) have been isolated and the contents of the pharmacologically most interesting compounds isopetasin (1b), neopetasin (2b), and petasin (3b), have been quantitatively determined. Additionally two of the basic alcohols petasol and isopetasol have been detected for the first time in small amounts in roots of P. hybridus.