Bildung der Thylakoidproteine während der Ergrünung einer vergilbten Mutante von Chlamydomonas reinhardi

Abstract

The thylakoid proteins of green, of bleached, and of regreening cells from the chromosomal, streptomycin-resistant mutant sr₃ of Chlamydomonas reinhardi have been analyzed by SDS-polyacrylamidegel electrophoresis. The prominent protein band I of thylakoids from green cells (m.w. approx. 67000) is characteristic for photosystem I-particles, whereas the major bands IIa, IIb and Iic (m.w. approx. 32000, 28000, 23000, respectively) are accumulated in photosystem II preparations. Even after purification by flotation in sucrose, several minor bands of glycoproteins can be detected in green thylakoids. In streptomycin-bleached cells the characteristic pattern of thylakoid proteins as well as the ribulose-1,5-diphosphate carboxylase are absent. During light-induced greening of such cells under conditions of suppressed cell division, the protein bands I and IIa, as well as chlorophyll and ribulose-1,5-diphosphate carboxylase became detectable after a lag of 24 h. The synthesis of the protein bands IIb and IIc, which are reported to be related to thylakoid stacking, starts even later, but continues longer.