Immobilisierte Enzyme und Prinzipien für ihre Anwendung bei analytischen und präparativen Verfahren

Abstract

On the basis of the physical and chemical properties of the catalytically active protein some selected methods for the covalent binding of enzymes to natural and synthetic polymers are presented.

Only a limited number of functional groups are able to react with the enzyme protein without destroying its catalytic activity. On the other hand there are many ways to build matrices bearing these groups. Some aspects referring to the kinetical characterisation of immobilized enzymes are discussed.

In most cases the application of insolubilized enzymes is still in its experimental stage. In the case of the glucose oxidase reaction the use of enzyme reactors as analytical tools is compared with their application for preparative reactions.

Considering basic economical aspects, some examples are presented in which the immobilized enzyme may be used more profitable than the soluble one. Reactions providing the isomers of optically active molecules are of special interest.