Immobilisierte Enzyme

5. Internationales Makromolekulares Symposium

Abstract

Waterinsoluble active enzyme derivatives can be prepared by covalent binding of enzymes to reactive polymers. An introduction is given into the development of these reactive polymer carriers. In particular own results are dealt with: Papain and other enzymes were immobilized with hydrophilic neutral resp. polyanionic carriers. The starting material for the neutral matrices was poly vinyl alcohol or poly acrolein. In the case of the poly anionic matrices macroreticu-lar copolymers were used synthesized by copolymerization of 3- or 4-isothiocyanatostyrene, acrylic acid, and divinylbenzene as cross-linking agent in different ratios. The binding ability of the reactive carriers for enzymes was investigated in dependance of some properties of the carrier as surface, content of reactive groups, and swellability. Some of the immobilized enzymes show shifted pH-optima, reduced K_M-values, and increased thermostability. Their enzymatic activity was constant for a prolonged period. Besides, an overview of some applications of immobilized enzymes is given.